Terminating substrates of DNA polymerases: synthesis and functional study

In order to study the mechanisms of DNA biosynthesis a number of modified nucleoside - substrates of DNA polymerases was synthesized. The absence of hydroxyl at 3'-position of ribose results in terminating properties of DNA biosynthesis of these analogues. A single step synthesis of triphosphat...

Full description

Saved in:
Bibliographic Details
Published inNucleic acids symposium series (1979) no. 18; pp. 117 - 120
Main Authors Dyatkina, N B, Arzumanov, A A
Format Journal Article
LanguageEnglish
Published England 1987
Subjects
DNA - biosynthesis
DNA - chemical synthesis
DNA-Directed DNA Polymerase - metabolism
Indicators and Reagents
Substrate Specificity
Online AccessGet full text

Cover

More Information
Summary:In order to study the mechanisms of DNA biosynthesis a number of modified nucleoside - substrates of DNA polymerases was synthesized. The absence of hydroxyl at 3'-position of ribose results in terminating properties of DNA biosynthesis of these analogues. A single step synthesis of triphosphates and alpha-thiotriphosphates of natural and 3'-modified 2'-deoxynucleosides is described.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0261-3166