Epidermal growth factor receptor monoclonal antibodies inhibit the growth of lung cancer cell lines

The ability of monoclonal antibody (MAb 108), an immunoglobulin G (IgG)2a against the epidermal growth factor receptor (EGF-R), to interact with lung cancer cell lines was investigated. 125I-EGF bound with high affinity to non-small-cell lung cancer (NSCLC) cells, and MAb 108 inhibited specific bind...

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Bibliographic Details
Published inJournal of the National Cancer Institute. Monographs no. 13; p. 117
Main Authors Lee, M, Draoui, M, Zia, F, Gazdar, A, Oie, H, Bepler, G, Bellot, F, Tarr, C, Kris, R, Moody, T W
Format Journal Article
LanguageEnglish
Published United States 1992
Subjects
Animals
Antibodies, Monoclonal
Carcinoma, Non-Small-Cell Lung - prevention & control
Dose-Response Relationship, Drug
Female
Lung Neoplasms - prevention & control
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasm Transplantation
Receptor, Epidermal Growth Factor - immunology
Receptor, Epidermal Growth Factor - physiology
Tumor Cells, Cultured
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Summary:The ability of monoclonal antibody (MAb 108), an immunoglobulin G (IgG)2a against the epidermal growth factor receptor (EGF-R), to interact with lung cancer cell lines was investigated. 125I-EGF bound with high affinity to non-small-cell lung cancer (NSCLC) cells, and MAb 108 inhibited specific binding of nine NSCLC cell lines in a dose-dependent manner (IC50 = 0.3-3 micrograms per ml). 125I-MAb 108 bound with high affinity (kd = 2 nM) to a single class of sites (Bmax = 70,000 per cell) using NSCLC neuroendocrine cell line NCI-H460. Specific 125I-MAb 108 binding was inhibited with high affinity by MAb 108 but not by a control antibody IgG using large-cell carcinoma cell line NCI-H1299. 125I-MAb 108 binding was not internalized at 37 degrees C using NSCLC neuroendocrine cell line NCI-H460 and adenocarcinoma cell line NCI-H23. Also, 1 microgram per ml of MAb 108 but not of a control IgG inhibited the clonal growth of NCI-H23 and squamous cell carcinoma cell line NCI-H157 in vitro. Also, MAb 108 inhibited xenograft formation of cell lines NCI-H460, NCI-H157, and NCI-H727 in nude mice in vivo. After a palpable tumor had formed using NCI-H460 cells, injection of 100 micrograms of MAb 108 (intraperitoneally three times weekly) inhibited xenograft volume in nude mice by approximately 50%. These data suggest that MAb 108 may interact with EGF receptors on lung cancer cell lines and inhibit NSCLC proliferation.
ISSN:1052-6773