Leukaemic B-cell maturation: studies on immunoglobulin secretion mediated by two signalling systems in vitro

The in vitro production and secretion of the immunoglobulins IgM, IgG, Kappa and lambda light chains by peripheral blood lymphocytes from chronic lymphocytic leukaemia patients was examined. The ELISA system was employed to quantitate the immunoglobulins secreted in the culture supernatants. Lipopol...

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Bibliographic Details
Published inCytobios Vol. 84; no. 336; p. 31
Main Authors Antosz, H, Koczkodaj, D, Kwiatkowska-Drabik, B, Wach, W
Format Journal Article
LanguageEnglish
Published England 1995
Subjects
B-Lymphocytes - cytology
B-Lymphocytes - immunology
B-Lymphocytes - secretion
Cell Differentiation - immunology
Enzyme-Linked Immunosorbent Assay
Humans
Immunoglobulin G - metabolism
Immunoglobulin kappa-Chains - metabolism
Immunoglobulin lambda-Chains - metabolism
Immunoglobulin M - metabolism
Immunoglobulins - biosynthesis
Immunoglobulins - metabolism
Immunophenotyping
Leukemia, B-Cell
Leukemia, Lymphocytic, Chronic, B-Cell
Lipopolysaccharides - pharmacology
Lymphocyte Activation - drug effects
Signal Transduction - immunology
Tetradecanoylphorbol Acetate - pharmacology
Tumor Cells, Cultured - cytology
Tumor Cells, Cultured - immunology
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Summary:The in vitro production and secretion of the immunoglobulins IgM, IgG, Kappa and lambda light chains by peripheral blood lymphocytes from chronic lymphocytic leukaemia patients was examined. The ELISA system was employed to quantitate the immunoglobulins secreted in the culture supernatants. Lipopolysaccharide and phorbol esters with CA++ ionophore were used as lymphocyte stimulators. The maturation of cultured lymphocytes was demonstrated by synthesis of cytoplasmic immunoglobulins and Ig secretions typical for plasma cells. In one case an isotype switch C mu for C gamma was observed.
ISSN:0011-4529