Telluromethionine in structural biochemistry

One of the fundamental problems in macromolecular crystallography is the availability of the suitable heavy-atom derivatives necessary to solve the phase problem. The ability to label a protein with a tellurium-containing amino acid (telluromethionine) at internal sites through the utilization of pr...

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Bibliographic Details
Published inSAAS bulletin, biochemistry and biotechnology Vol. 8; p. 29
Main Authors Boles, J O, Lebioda, L, Dunlap, R B, Odom, J D
Format Journal Article
LanguageEnglish
Published United States 1995
Subjects
Crystallization
Crystallography, X-Ray
Escherichia coli - enzymology
Magnetic Resonance Spectroscopy
Methionine - analogs & derivatives
Methionine - analysis
Methotrexate - metabolism
NADP - metabolism
Tellurium - analysis
Tetrahydrofolate Dehydrogenase - chemistry
Tetrahydrofolate Dehydrogenase - metabolism
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Summary:One of the fundamental problems in macromolecular crystallography is the availability of the suitable heavy-atom derivatives necessary to solve the phase problem. The ability to label a protein with a tellurium-containing amino acid (telluromethionine) at internal sites through the utilization of protein biosynthesis supplies x-ray crystallographers a convenient phasing vehicle and nuclear magnetic resonance (NMR) spectroscopists an internal probe with which to study structure/function relationships via Te-125 NMR spectroscopy. In this communication we demonstrate the partial incorporation of telluromethionine into E. coli dihydrofolate reductase (DHFR) with no apparent perturbations to activity or substrate binding. Enzyme containing two moles TeMet exhibited a specific activity of 42 units/mg and a 1:1 binding ratio with methotrexate.
ISSN:1052-6781