Influence of the incubation of cells with zinc and lithium ions on GVH reactivity of cells and on their ability to form haemopoietic colonies

• Published in: Folia biologica Vol. 32; no. 2; pp. 81 - 90
• Main Authors: Hrubá, A, Paluska, E, Chudomel, V
• Format: Journal Article
• Language: English
• Published: Czech Republic 1986
• Subjects: Animals; Bone Marrow - drug effects; Bone Marrow Transplantation; Colony-Forming Units Assay; Female; Graft vs Host Reaction - drug effects; Hematopoiesis - drug effects; In Vitro Techniques; Lithium - pharmacology; Male; Mice; Mice, Inbred Strains; Spleen - drug effects; Spleen - transplantation; Transplantation, Homologous; Zinc - pharmacology
• ISSN: 0015-5500

Mouse spleen and bone marrow cells were incubated for 2 h with ZnCl2 and Li2SO4 at different concentrations and tested for the ability to evoke the graft-versus-host reactions (GVHR) and to form pluripotent haemopoietic colonies. ZnCl2 at concentrations 5 X 10(-6) to 5 X 10(-4) M inhibited the regional GVHR. At a concentration of 5 X 10(-6) ZnCl2 also inhibited the ability to elicit the systemic GVHR in irradiated mice. Haemopoiesis was stimulated in cells incubated with ZnCl2 at concentration 5 X 10(-6) M, it was inhibited after incubation of A/01a cells with 5 X 10(-4) M ZnCl2, whereas cells from non-inbred ICR mice were stimulated by the latter concentration of ZnCl2 for haemopoiesis. Li2SO4 inhibited the ability to induce the regional GVHR at concentrations of 5 X 10(-3) and 10(-2) M, had no effect on survival of mice during the systemic GVHR and exerted rather an inhibitory effect on haemopoiesis.