Quantitating histamine in the saliva and salivary glands of two Palaearctic blackfly species (Diptera: Simuliidae)

Saliva was collected from females of Boophthora erythrocephala (Simulium erythrocephalum) and Wilhelmia lineata (S. lineatum) as they were feeding on a saline-ATP solution through an artificial silicone membrane. The histamine content of the saliva was measured by high-performance liquid chromatogra...

Full description

Saved in:
Bibliographic Details
Published inTropical medicine and parasitology Vol. 39; no. 4; p. 309
Main Author Wirtz, H P
Format Journal Article
LanguageEnglish
Published Germany 01.12.1988
Subjects
Animals
Chromatography, High Pressure Liquid
Feeding Behavior
Female
Histamine - analysis
Saliva - analysis
Salivary Glands - analysis
Simuliidae - analysis
Simuliidae - physiology
Online AccessGet more information

Cover

More Information
Summary:Saliva was collected from females of Boophthora erythrocephala (Simulium erythrocephalum) and Wilhelmia lineata (S. lineatum) as they were feeding on a saline-ATP solution through an artificial silicone membrane. The histamine content of the saliva was measured by high-performance liquid chromatography (HPLC). The amount of histamine salivated by B. erythrocephala (2.9 ng/individual when feeding to repletion) was more than four times that released by W. lineata (0.7 ng/individual). For both species, the quantity of histamine salivated increased with duration of feeding and partially with the age of females. The proportion of histamine retaines by in vitro fed W. lineata averaged 36.1% W. lineata females engorged on saline-ATP solution, or stimulated for feeding with the same medium, produced significantly more hist-amine than died unstimulated flies.
ISSN:0177-2392