Experimental study of effects of nm23-H1 gene transfection on the change of PKA activity in human high-metastasis large cell lung cancer cell line L9981

• Published in: Zhongguo fei ai za zhi Vol. 7; no. 2; p. 91
• Main Authors: Li, Dingbiao, Zhou, Qinghua, Wang, Yanping, Zhu, Wen, Chen, Xiaohe, Yang, Junjie, Liu, Lunxu, Fu, Junke, Nie, Qiang, Li, Yin
• Format: Journal Article
• Language: Chinese
• Published: China Chinese Anti-Cancer Association Chinese Antituberculosis Association 20.04.2004
• Subjects: Kinases; Lung cancer; Metastasis; Signal transduction
• ISSN: 1009-3419; 1999-6187
• DOI: 10.3779/cjlc.v7i2.2520

To explore the effects of nm23-H1 gene transfection and forskolin on PKA activity in human high-metastasis large cell lung cancer cell line L9981. Three cell lines, primary human large cell lung cancer cell line (L9981), vector transfection cell line (L9981-pLXSN) and nm23-H1 gene transfection cell line (L9981-nm23-H1-pLXSN), were treated with PKA activator forskolin. The PKA activity at different time points after treatment with forskolin was detected in the three lung cancer cell lines by radioimmunological method with SignaTECT cAMP-dependent PKA assay system. (1) Before forskolin treatment, the activity of PKA of L9981-nm23-H1-pLXSN was remarkably higher than those of L9981 and L9981-pLXSN (P < 0.01), but no significant difference in the PKA activity was observed between L9981 and L9981-pLXSN (P > 0.05). (2)The PKA activity was remarkably increased in all the three lung cancer cell lines after treatment with different concentration of forskolin (P < 0.01), and up to the highest level at the concentration