In vitro studies of the fixation of DNA, nucleoprotamine, nucleohistone and proteins
In view of improving fixation and embedding for thin sections of nuclear material we investigated the crosslinking of selected proteins, nucleic acid and nucleoproteins by observing the eventual gelation of solutions of these substances, which might be produced by current cytological fixatives. For...
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| Published in | European journal of cell biology Vol. 25; no. 1; p. 1 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Germany
01.08.1981
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| Subjects | |
| Online Access | Get more information |
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| Abstract | In view of improving fixation and embedding for thin sections of nuclear material we investigated the crosslinking of selected proteins, nucleic acid and nucleoproteins by observing the eventual gelation of solutions of these substances, which might be produced by current cytological fixatives. For those combinations where gelation occurs, we give thresholds of minimum concentrations required. We discuss the consequences of these findings for the preservation of thin sectioned nuclear structures within cells. |
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| AbstractList | In view of improving fixation and embedding for thin sections of nuclear material we investigated the crosslinking of selected proteins, nucleic acid and nucleoproteins by observing the eventual gelation of solutions of these substances, which might be produced by current cytological fixatives. For those combinations where gelation occurs, we give thresholds of minimum concentrations required. We discuss the consequences of these findings for the preservation of thin sectioned nuclear structures within cells. |
| Author | Wunderli, H Kellenberger, E Stauffer, E Carlemalm, E Kellenberger, C |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/6793368$$D View this record in MEDLINE/PubMed |
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| Snippet | In view of improving fixation and embedding for thin sections of nuclear material we investigated the crosslinking of selected proteins, nucleic acid and... |
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| SubjectTerms | Chromatin - ultrastructure Chromosomal Proteins, Non-Histone DNA Fixatives Gels Glutaral Histones Magnesium Osmium Tetroxide Protamines |
| Title | In vitro studies of the fixation of DNA, nucleoprotamine, nucleohistone and proteins |
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