In vitro studies of the fixation of DNA, nucleoprotamine, nucleohistone and proteins

In view of improving fixation and embedding for thin sections of nuclear material we investigated the crosslinking of selected proteins, nucleic acid and nucleoproteins by observing the eventual gelation of solutions of these substances, which might be produced by current cytological fixatives. For...

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Bibliographic Details
Published inEuropean journal of cell biology Vol. 25; no. 1; p. 1
Main Authors Kellenberger, E, Carlemalm, E, Stauffer, E, Kellenberger, C, Wunderli, H
Format Journal Article
LanguageEnglish
Published Germany 01.08.1981
Subjects
Chromatin - ultrastructure
Chromosomal Proteins, Non-Histone
DNA
Fixatives
Gels
Glutaral
Histones
Magnesium
Osmium Tetroxide
Protamines
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Summary:In view of improving fixation and embedding for thin sections of nuclear material we investigated the crosslinking of selected proteins, nucleic acid and nucleoproteins by observing the eventual gelation of solutions of these substances, which might be produced by current cytological fixatives. For those combinations where gelation occurs, we give thresholds of minimum concentrations required. We discuss the consequences of these findings for the preservation of thin sectioned nuclear structures within cells.
ISSN:0171-9335