Changes in Ca++-ATPase activity of rat myocyte under cardioplegia

Ischemic changes of cardiac muscle under cardioplegia must be evaluated at a subcellular level from functional aspects using cytochemical procedures as well as from morphological aspects. In this study, kinetics of Ca++-ATPase activity of rat cardiac myocytes under GIK-cardioplegia was estimated usi...

Full description

Saved in:
Bibliographic Details
Published inNihon Geka Gakkai zasshi Vol. 88; no. 6; p. 755
Main Authors Satoh, S, Kawai, T, Kitaura, K, Oga, K, Oka, T
Format Journal Article
LanguageJapanese
Published Japan 01.06.1987
Subjects
Actin Cytoskeleton - enzymology
Animals
Calcium-Transporting ATPases - metabolism
Glucose - administration & dosage
Heart Arrest, Induced
Insulin - administration & dosage
Microscopy, Electron
Mitochondria, Heart - enzymology
Myocardium - cytology
Myocardium - ultrastructure
Potassium - administration & dosage
Rats
Rats, Inbred Strains
Sarcoplasmic Reticulum - enzymology
Online AccessGet more information

Cover

More Information
Summary:Ischemic changes of cardiac muscle under cardioplegia must be evaluated at a subcellular level from functional aspects using cytochemical procedures as well as from morphological aspects. In this study, kinetics of Ca++-ATPase activity of rat cardiac myocytes under GIK-cardioplegia was estimated using a new one-step lead citrate method reported by Ando et al. The Ca++-ATPase activity of mitochondria in myocytes which had temporally increased at 15 minutes after initiation of cardioplegia, showed a slight decrease at 30 minutes and then almost disappeared at 60 minutes. At 60 minutes after the cardioplegia, the Ca++-ATPase activities of the sarcoplasmic reticula and myofilaments were well preserved and electromicroscopic findings of the myocytes remained normal. Thus, changes of Ca++-ATPase activity of mitochondria are considered to reflect ischemic damage of myocyte sharply, proceeding morphological changes in ultrastructure.
ISSN:0301-4894