Obtaining of pure transferrins D, M and R from equine serum and determination of transferrin level in relation to phenotype

By the method of precipitation with Rivanol (2-ethoxy-6,9-diaminoacridine lactate) and ammonium sulphate followed by chromatography on DEAE cellulose three genetic variants of transferrin were purified from equine serum: D, M and R. Their molecular mass determined in this study was 80 000, and it wa...

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Bibliographic Details
Published inActa physiologica Polonica Vol. 35; no. 5-6; p. 529
Main Authors Didkowski, S, Kaminski, M, Kerjan, P, Tomaszewska-Guszkiewicz, K, Zurkowski, M
Format Journal Article
LanguageEnglish
Published Poland 01.09.1984
Subjects
Amino Acids - analysis
Animals
Electrophoresis, Agar Gel
Electrophoresis, Polyacrylamide Gel
Genetic Variation
Homozygote
Horses - blood
Immunoelectrophoresis
Molecular Weight
Phenotype
Polymorphism, Genetic
Transferrin - genetics
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Summary:By the method of precipitation with Rivanol (2-ethoxy-6,9-diaminoacridine lactate) and ammonium sulphate followed by chromatography on DEAE cellulose three genetic variants of transferrin were purified from equine serum: D, M and R. Their molecular mass determined in this study was 80 000, and it was identical for all three variants, which differed slightly in their amino acid composition. The protein level was determined in the serum of 535 two-year-old thoroughbred English horses by the method of rocket immunoelectrophoresis using antibodies obtained against three transferrins. The individual variability of the protein level in horses of the same phenotype was fairly high (variability index 9-15%). No differences were observed in the transferrin level related to sex. It was found that the presence of D, F and H alleles was connected with a higher serum transferrin level, while O and R alleles were connected with a lower level.
ISSN:0044-6033