A tissue culture assay for diphtheria toxicity testing

A tissue culture assay system in microtitre plates using a Vero cell line has been compared with the traditional guinea pig skin reaction test to detect residual toxicity during the diphtheria toxin detoxification procedure. A standard batch of untreated diphtheria toxin was used as a reference stan...

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Bibliographic Details
Published inDevelopments in biological standardization Vol. 64; p. 33
Main Authors Abreo, C B, Stainer, D W
Format Journal Article
LanguageEnglish
Published Switzerland 1986
Subjects
Animals
Cell Survival - drug effects
Cells, Cultured
Diphtheria Toxin - toxicity
Dose-Response Relationship, Drug
Guinea Pigs
In Vitro Techniques
Neutralization Tests
Skin Tests
Vero Cells
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Summary:A tissue culture assay system in microtitre plates using a Vero cell line has been compared with the traditional guinea pig skin reaction test to detect residual toxicity during the diphtheria toxin detoxification procedure. A standard batch of untreated diphtheria toxin was used as a reference standard in each of the assays. The tissue culture assay proved to be sensitive, specific, economical and reproducible and offers an alternative to the use of guinea pigs in testing for any residual toxicity in diphtheria toxoid samples.
ISSN:0301-5149