New flow cytometric technologies for the 21st century

• Published in: Human cell : official journal of Human Cell Research Society Vol. 10; no. 1; p. 3
• Main Authors: Roslaniec, M C, Bell-Prince, C S, Crissman, H A, Fawcett, J J, Goodwin, P M, Habbersett, R, Jett, J H, Keller, R A, Martin, J C, Marrone, B L, Nolan, J P, Park, M S, Sailer, B L, Sklar, L A, Steinkamp, J A, Cram, L S
• Format: Journal Article
• Language: English
• Published: Japan 01.03.1997
• Subjects: Chromosomes, Human; DNA - analysis; DNA Fragmentation; Flow Cytometry - methods; Flow Cytometry - trends; Fluorescent Dyes; Forecasting; Humans; Karyotyping
• ISSN: 0914-7470; 1749-0774

The envelope that defines the limits within which flow cytometry was developed is being rapidly expanded. For example: detection sensitivity has been extended to single molecules, the size range of "particle" analysis now extends from DNA fragments to plankton (1,000.+ microns), cell and chromosome sorting rates are being increased dramatically by using inactivation procedures (50,000 per second versus 2,000 per second), rapid kinetic flow cytometry enables real-time analysis of molecular assembly and cell function in the sub-second time domain, the lifetime of a fluorochrome bound to a single cell can be measured with nsec precision, and classical karyotype information (cell to cell heterogeneity) can be determined in a flow based system. These frontiers have greatly expanded the range of new and exciting flow cytometric based biomedical applications. New enabling technologies have provided the means to measure DNA cleavage by the structure-specific nuclease, human Flap Endonuclease (FEN-1), in the 300 msec time frame. Phase sensitive measurements and fluorescence lifetime are proving to be major advances for understanding molecular environments that change with, for example, the process of apoptosis. The ability to detect single fluorescent molecules has been applied to the analysis of DNA fragments obtained from enzymatic digestion of lambda DNA. This technology is being used to rapidly and very accurately size DNA fragments for the human genome project. Optical chromosome selection is a faster, better, less complex approach to chromosome sorting. This method is based on the induction of specific damage to the DNA of selected chromosomes. Lastly, the miniaturization of a single cell fractionator has made it possible to perform single cell flow cytogenetics.