High performance liquid chromatography of nucleic acids and the related compounds on a fluorinated silica gel column

Separations of nucleic acids and the related compounds were investigated by HPLC on a new fluorinated bonded silica gel column. Polyadenylate (Poly (A)) enzymatic partial hydrolysate sample and the mixture of various polynucleotide samples were sufficiently separated by the reversed-phase mode using...

Full description

Saved in:
Bibliographic Details
Published inNucleic acids symposium series (1979) no. 27; pp. 27 - 28
Main Authors Itoh, H, Kinoshita, T, Nimura, N
Format Journal Article
LanguageEnglish
Published England 1992
Subjects
Chromatography, High Pressure Liquid - instrumentation
Fluorocarbons - chemistry
Polynucleotides - isolation & purification
RNA, Transfer - isolation & purification
Silica Gel
Silicon Dioxide - chemistry
Online AccessGet full text

Cover

More Information
Summary:Separations of nucleic acids and the related compounds were investigated by HPLC on a new fluorinated bonded silica gel column. Polyadenylate (Poly (A)) enzymatic partial hydrolysate sample and the mixture of various polynucleotide samples were sufficiently separated by the reversed-phase mode using a gradient elution with aqueous ammonium acetate/acetonitrile system. Mixed-mode separation on the fluorinated bonded phase coated with a tert-alkylammonium salt was also examined for the separation of the various polynucleotides including tRNAs.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0261-3166