DNA ploidy of human breast cancer

Ploidy was determined on 663 resectable primary tumors from untreated patients. Nuclei obtained by mechanical disaggregation of frozen tissue were stained with propidium iodide and analysed in a FACS IV. Aneuploidy was detected in 73% of cases. It was not significantly related to nodal involvement o...

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Bibliographic Details
Published inAnalytical cellular pathology Vol. 1; no. 4; p. 215
Main Authors Del Bino, G, Silvestrini, R, Zucconi, M R, Marchese, G, Valentinis, B, Di Fronzo, G, Andreola, S
Format Journal Article
LanguageEnglish
Published Netherlands 01.08.1989
Subjects
Adult
Aged
Aged, 80 and over
Breast Neoplasms - chemistry
Breast Neoplasms - genetics
Breast Neoplasms - pathology
Cell Fractionation - methods
DNA, Neoplasm - analysis
Female
Freezing
Humans
Lymphatic Metastasis
Middle Aged
Ploidies
Receptors, Estrogen - analysis
Receptors, Progesterone - analysis
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Summary:Ploidy was determined on 663 resectable primary tumors from untreated patients. Nuclei obtained by mechanical disaggregation of frozen tissue were stained with propidium iodide and analysed in a FACS IV. Aneuploidy was detected in 73% of cases. It was not significantly related to nodal involvement or tumor size, although the highest frequencies were observed in large tumors (88%) or with more than 10 positive nodes (77%). Aneuploidy was more frequently observed in ductal infiltrating (81%) than in lobular histology and in tumors lacking both progesterone and estrogen receptors (85%). Analysis of ploidy in primary and synchronous lymph node metastases from the same patient showed a high agreement rate (90%) of DNA patterns simply defined as diploid or aneuploid. However, differences in DNA stemlines and DNA indices between the two synchronous lesions from the same patient were a rather frequent event.
ISSN:0921-8912