Increased expression of the monocyte differentiation antigen CD14 in extrinsic allergic alveolitis

Expression of the CD14 antigen was studied on alveolar macrophages in extrinsic allergic alveolitis (EAA), using immunocytochemistry and cytometry. Compared to control donors, EAA patients had higher percentages of My4 positive cells (40 versus 22%), and the antigen density was fourfold higher (410...

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Published inMonaldi archives for chest disease Vol. 48; no. 6; p. 607
Main Authors Pforte, A, Schiessler, A, Gais, P, Beer, B, Ströbel, M, Ehlers, M, Schütt, C, Ziegler-Heitbrock, H W
Format Journal Article
LanguageEnglish
Published Italy 01.12.1993
Subjects
Adult
Aged
Alveolitis, Extrinsic Allergic - immunology
Antigens, CD - analysis
Antigens, Differentiation, Myelomonocytic - analysis
Female
Humans
Lipopolysaccharide Receptors
Macrophages, Alveolar - immunology
Male
Middle Aged
Monocytes - immunology
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Summary:Expression of the CD14 antigen was studied on alveolar macrophages in extrinsic allergic alveolitis (EAA), using immunocytochemistry and cytometry. Compared to control donors, EAA patients had higher percentages of My4 positive cells (40 versus 22%), and the antigen density was fourfold higher (410 versus 92 channels). Levels of soluble CD14 (sCD14) in serum were found to be increased in EAA patients with an average of 4.6 +/- 1.5 micrograms.ml-1 compared to 3.2 +/- 0.7 micrograms.ml-1 in controls. Follow-up of patients with antigen avoidance revealed a concomitant decrease of CD14 staining of alveolar macrophages (AMs) and of sCD14 in serum, whilst allergen exposure induces both parameters. These data are consistent with the concept that antigen contact upregulates CD14 expression on AMs in EAA, followed by shedding and increase of sCD14 in serum.
ISSN:1122-0643