Kinetic parameters of GABA receptor binding by rat adenohypophyseal membranes under conditions of modelling various corticosteroid and ACTH levels in the body

• Published in: Problemy ėndokrinologii Vol. 39; no. 6; p. 43
• Main Authors: Mishunina, T M, Kononenko, V Ia
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.11.1993
• Subjects: Adrenal Glands - physiology; Adrenocorticotropic Hormone - blood; Animals; Cell Membrane - physiology; Corticosterone - blood; gamma-Aminobutyric Acid - metabolism; Kinetics; Male; Models, Biological; Pituitary Gland, Anterior - metabolism; Rats; Rats, Wistar; Receptors, GABA - metabolism
• ISSN: 0375-9660
• DOI: 10.14341/probl11949

Kinetic parameters of 14C-GABA specific binding by rat adenohypophyseal plasma membranes were studied in experiments on modelling various corticosteroid and ACTH levels in animal body. A single hydrocortisone injection did not change K alpha for high- and low-affinity GABA receptors, the number of the former (Bmax) increasing in this case. Repeated hydrocortisone injections were associated with K alpha reduction for high-affinity GABA receptors and a noticeable increase of K alpha for low-affinity receptors, with their number reducing. ACTH injection did not change the kinetic parameters of GABA binding with receptors. The number of high-affinity GABA receptors and their affinity reduced after removal of adrenals whereas the number of low-affinity receptors in this case was increasing. A single hydrocortisone injection to adrenalectomized rats had a normalizing effect on adenohypophyseal GABA receptors. Analysis of the results and changes in blood hormonal levels indicated that affinity changes in high-affinity receptors and changed number of low-affinity adenohypophyseal GABA receptors correlated with changes in ACTH and hydrocortisone changes.