Phenylalanyl-tRNA synthetase from Thermus thermophilus can attach two molecules of phenylalanine to tRNA(Phe)

Phenylalanyl-tRNA synthetase from the extreme thermophilic bacterium Thermus thermophilus can incorporate more than one molecule of phenylalanine into the tRNA(Phe). It is shown that the 'hyperaminoacylated' tRNA(Phe) is the bis-2',3'-O-phenylalanyl-tRNA(Phe), and its formation i...

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Bibliographic Details
Published inFEBS letters Vol. 311; no. 3; pp. 192 - 194
Main Authors Stepanov, V G, Moor, N A, Ankilova, V N, Lavrik, O I
Format Journal Article
LanguageEnglish
Published England 26.10.1992
Subjects
Autoradiography - methods
Carbon Radioisotopes
Chromatography, Thin Layer
Kinetics
Phenylalanine-tRNA Ligase - metabolism
Protein Binding
Radioisotope Dilution Technique
RNA, Transfer, Phe - isolation & purification
RNA, Transfer, Phe - metabolism
Thermus thermophilus - enzymology
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Summary:Phenylalanyl-tRNA synthetase from the extreme thermophilic bacterium Thermus thermophilus can incorporate more than one molecule of phenylalanine into the tRNA(Phe). It is shown that the 'hyperaminoacylated' tRNA(Phe) is the bis-2',3'-O-phenylalanyl-tRNA(Phe), and its formation is typical for the thermophilic enzyme but does not occur for E. coli phenylalanyl-tRNA synthetase under the same conditions.
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ISSN:0014-5793
DOI:10.1016/0014-5793(92)81099-8