A technique for isolation of bone marrow cells using hydroxyethyl starch (HES) sedimentation agent

Various methods have been investigated to concentrate hemopoietic stem cells before in vitro treatment and cryopreservation. Each procedure requires various degrees of cell manipulation; moreover, the extent of purification of the stem cell fraction depends on the further use of bone marrow. Fifty f...

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Bibliographic Details
Published inHaematologica (Roma) Vol. 76 Suppl 1; p. 7
Main Authors Montuoro, A, De Rosa, L, Del Monte, C, Blandino, F, Pandolfi, A, Lanti, T, De Laurenzi, A
Format Journal Article
LanguageEnglish
Published Italy 01.03.1991
Subjects
Blood Group Incompatibility
Blood Sedimentation - drug effects
Bone Marrow - pathology
Bone Marrow Cells
Bone Marrow Transplantation - methods
Cell Count
Cell Separation - methods
Hemagglutinins
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells
Humans
Hydroxyethyl Starch Derivatives - pharmacology
Isoantibodies
Leukocytes, Mononuclear - transplantation
Transplantation, Autologous
Transplantation, Homologous
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Summary:Various methods have been investigated to concentrate hemopoietic stem cells before in vitro treatment and cryopreservation. Each procedure requires various degrees of cell manipulation; moreover, the extent of purification of the stem cell fraction depends on the further use of bone marrow. Fifty four bone marrows were treated with hydroxyethyl starch (HES) to isolate the mononuclear cell population. The manipulation with HES permitted to concentrate marrow stem cells in a small volume with removal of unwanted granulocytes, red blood cells and the donor isohemagglutinins in the major ABH incompatibility. We describe here a manual method which permits the transfusion of a very few number of red cells without depletion of progenitor cells and thus delay of engraftment.
ISSN:0390-6078
1592-8721