Human cord blood progenitors: kinetics, regulation and their use for hemopoietic reconstitution

The high number of cord blood (CB) hematopoietic progenitors made possible CB transplantation in children with hematologic diseases. We collected and cryopreserved CB from siblings of 11 hematologic pediatric patients for possible transplant. The number of BFU-E+CFU-GEMM and CFU-GM was adequate for...

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Published inBone marrow transplantation (Basingstoke) Vol. 12 Suppl 1; pp. 84 - 86
Main Authors Gabutti, V, Timeus, F, Ramenghi, U, Saracco, P, Fassio, D, Giubellino, M C, Miniero, R, Madon, E
Format Journal Article
LanguageEnglish
Published England 1993
Subjects
Blood Cell Count
Blood Component Transfusion
Cells, Cultured
Child
Cytokines - pharmacology
Fetal Blood - cytology
Hematologic Diseases - genetics
Hematologic Diseases - therapy
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - drug effects
Humans
Infant, Newborn
Nuclear Family
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Summary:The high number of cord blood (CB) hematopoietic progenitors made possible CB transplantation in children with hematologic diseases. We collected and cryopreserved CB from siblings of 11 hematologic pediatric patients for possible transplant. The number of BFU-E+CFU-GEMM and CFU-GM was adequate for engraftment. However the possibility of CB transplantation in adolescents and adults is limited by the amount of CB that can be collected. We studied the possibility of expanding the pool of CB hematopoietic progenitors by preincubation of CB CD 34+ cells with several cytokines. After pre-exposition to rhIL-3, IL-1 beta, rIL-6, GM-CSF and stem cell factor (SCF) and cloning with SCF the number of hematopoietic progenitors increased 14-18 fold compared to basal values.
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ISSN:0268-3369
1476-5365