Rapid diagnosis of experimental tumor by FITC-Con A lectin--a comparative study of smear and section specimens

FITC-Con A staining as a rapid diagnostic method for tumor cells was applied to the tumors smeared on glass slide and section specimens to evaluate the reactivity with FITC-Con A. Good staining results were obtained in smear specimens with clear fluorescence on the membrane of tumor cells. Con A and...

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Bibliographic Details
Published inGan to kagaku ryoho Vol. 15; no. 4 Pt 1; p. 649
Main Authors Sasaki, J, Sawamura, D, Kitagawa, M, Ahsan, C R, Lü, C, Masumori, J
Format Journal Article
LanguageJapanese
Published Japan 01.04.1988
Subjects
Animals
Colonic Neoplasms - pathology
Concanavalin A - analogs & derivatives
Fluorescein-5-isothiocyanate - analogs & derivatives
Fluoresceins
Mice
Mice, Inbred BALB C
Microtomy
Specimen Handling - methods
Staining and Labeling - methods
Tumor Cells, Cultured
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Summary:FITC-Con A staining as a rapid diagnostic method for tumor cells was applied to the tumors smeared on glass slide and section specimens to evaluate the reactivity with FITC-Con A. Good staining results were obtained in smear specimens with clear fluorescence on the membrane of tumor cells. Con A and LCH lectins bound well with tumor cells to produce strong fluorescence in comparison with PEA and DBA. It indicates that tumor cells expressed dominantly the receptors of alpha-D-glucose and alpha-D-mannose sugar chain on the membrane of tumor cells. From these results it was concluded that FITC-Con A staining method applied to smear specimens is more advantageous in the rapidity and the simplicity for tumor cell diagnosis than section specimen method.
ISSN:0385-0684