Non-selective inhibition of mammalian protein kinases by flavinoids in vitro

In vitro, the flavinoids quercetin, morin, and rutin produced comparable inhibition of protein kinase C prepared from bovine brain, the epidermal growth factor (EGF)-stimulated tyrosine specific protein kinase associated with A431 cell membranes, and the cAMP-stimulated protein kinase activity assoc...

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Bibliographic Details
Published inResearch communications in chemical pathology and pharmacology Vol. 56; no. 1; p. 75
Main Authors End, D W, Look, R A, Shaffer, N L, Balles, E A, Persico, F J
Format Journal Article
LanguageEnglish
Published United States 01.04.1987
Subjects
Adenosine Triphosphate - metabolism
Animals
Cell Line
Electrophoresis, Polyacrylamide Gel
Epidermal Growth Factor - pharmacology
Flavonoids - pharmacology
Phosphoproteins - metabolism
Phosphorylation
Protein Kinase C - antagonists & inhibitors
Protein Kinase Inhibitors
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Summary:In vitro, the flavinoids quercetin, morin, and rutin produced comparable inhibition of protein kinase C prepared from bovine brain, the epidermal growth factor (EGF)-stimulated tyrosine specific protein kinase associated with A431 cell membranes, and the cAMP-stimulated protein kinase activity associated with rat brain tubulin. Concentrations producing 50% inhibition (IC50's) were in the 10 microM range. These findings demonstrate the lack of specificity of the flavinoids toward cyclic nucleotide-dependent vs cyclic nucleotide-independent protein kinases.
ISSN:0034-5164