New methodology for the synthesis of 2'(3')-O-aminoacyl oligoribonucleotides related to the 3'-terminus of aa-tRNA

A new synthetic approach to the title compounds is reported. It is based on the phosphotriester methodology and uses a unique combination of protecting groups (Fmoc and Ph for the aglycons; DMT, CPTr and Mthp for the carbohydrate hydroxy functions; 2-CIPh for the phosphodiester bonds; BPOC for amino...

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Bibliographic Details
Published inNucleic acids symposium series (1979) no. 18; pp. 285 - 288
Main Authors Hagen, M D, Scalfi-Happ, C, Happ, E, Chládek, S
Format Journal Article
LanguageEnglish
Published England 1987
Subjects
Indicators and Reagents
Oligoribonucleotides - chemical synthesis
RNA, Transfer, Amino Acyl - chemical synthesis
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Summary:A new synthetic approach to the title compounds is reported. It is based on the phosphotriester methodology and uses a unique combination of protecting groups (Fmoc and Ph for the aglycons; DMT, CPTr and Mthp for the carbohydrate hydroxy functions; 2-CIPh for the phosphodiester bonds; BPOC for amino acid). This enables selective deprotection of the blocked oligonucleotide intermediates in two steps (oximate and acid treatments) to yield the 2'(3')-O-aminoacyl oligoribonucleotides suitable for biochemical investigations.
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ISSN:0261-3166