Polyoma antigen driven T lymphocytes with antitumor and DTH activity in CBA mice

• Published in: Neoplasma Vol. 35; no. 3; pp. 271 - 278
• Main Authors: Ochałek, T, Cadel, Z
• Format: Journal Article
• Language: English
• Published: Slovakia 1988
• Subjects: Animals; Antigens, Neoplasm - immunology; Female; Hypersensitivity, Delayed - immunology; Immunization, Passive; Interleukin-2 - immunology; Male; Mice; Mice, Inbred CBA; Neoplasm Transplantation; T-Lymphocytes - immunology; T-Lymphocytes - transplantation; Tumor Virus Infections - immunology; Tumor Virus Infections - pathology; Tumor Virus Infections - therapy
• ISSN: 0028-2685

T lymphocytes isolated from a bulk culture of splenocytes exposed in vitro to polyoma TAA and IL-2, taken from CBA mice with transplanted polyoma tumors, exhibited both antitumor and DTH activity to polyoma TAA, associated with the Lyt 1+ phenotype, and also showed an increased affinity to TAA. The antitumor efficacy of adoptive immunotherapy, consisting in the i.p. administration of these TAA-driven lymphocytes, showed time and IL-2 dependence, while transferred cells were capable of eliciting DTH independently of IL-2. The suppression of tumor growth observed in 70% of the animals in the optimal variant seems to result from the cooperation of transferred lymphocytes with the recipients own lymphocytes.