Immunological evaluation of splenectomy in tumor-bearing mice

The effect of splenectomy upon neoplastic outgrowth was examined after inoculation of methylcholanthrene-induced C3H/He murine tumors. Three days or 20 days after tumor inoculation, splenectomy resulted in significant retardation of tumor growth when compared with sham operation, while splenectomy 6...

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Bibliographic Details
Published inNihon Geka Gakkai zasshi Vol. 84; no. 9; p. 987
Main Authors Yamagishi, H, Naito, K, Maeda, Y, Kurioka, H, Kobayashi, M, Fujimori, C, Iguchi, K, Tanaka, T, Hashimoto, I
Format Journal Article
LanguageJapanese
Published Japan 01.09.1983
Subjects
Animals
Cell Transformation, Neoplastic
Cytotoxicity, Immunologic
Immunoglobulins - analysis
Male
Mice
Mice, Inbred Strains
Neoplasms, Experimental - immunology
Spleen - immunology
Splenectomy
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Summary:The effect of splenectomy upon neoplastic outgrowth was examined after inoculation of methylcholanthrene-induced C3H/He murine tumors. Three days or 20 days after tumor inoculation, splenectomy resulted in significant retardation of tumor growth when compared with sham operation, while splenectomy 6, 9, 15 days after tumor inoculation did not alter the tumor outgrowth. These results suggest that spleen might have immunologically negative element in early or late stage of tumor burden. In fact, spleen cells from mice bearing MCA-F tumors for 3 days or 30 days nonspecifically facilitated the tumor outgrowth in Winn assay. The non-specific tumor-enhancing cells were radioresistant (700 rads), capable of phagocytizing carbonyl-iron and adherent to plastic dish suggesting those were tumor enhancing macrophages. On the other hand, spleen cells from tumor-bearing mice for 9 to 15 days specifically reduced the tumor growth in Winn assay, and those cytotoxic cells were radio-sensitive (700 rads) T cell population.
ISSN:0301-4894