Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein

• Published in: Folia biologica Vol. 36; no. 1; pp. 41 - 50
• Main Authors: Bazil, V, Stefanová, I, Hilgert, I, Kristofová, H, Vanĕk, S, Horejsí, V
• Format: Journal Article
• Language: English
• Published: Czech Republic 1990
• Subjects: Animals; Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - immunology; Antibody Specificity; Antigens, Differentiation - immunology; Antigens, Surface - immunology; Blotting, Western; CD11 Antigens; CD18 Antigens; Cytotoxicity, Immunologic - immunology; Electrophoresis, Polyacrylamide Gel; Female; Humans; Killer Cells, Natural - immunology; Leukocytes - immunology; Lymphocyte Function-Associated Antigen-1; Mice; Mice, Inbred BALB C; Receptors, Leukocyte-Adhesion - immunology
• ISSN: 0015-5500

Six monoclonal antibodies were prepared that recognize a broadly expressed antigen composed of noncovalently associated 95 kDa and 180 kDa subunits. Antibodies MEM-25, MEM-83 and MEM-95 are shown to react with a determinant(s) present on the isolated larger subunit identified as the CD11a glycoprotein, MEM-48 reacts with the isolated smaller subunit which is identical to the CD18 glycoprotein. Antibodies MEM-30 and MEM-94 recognize probably a determinant in the CD11a/CD18 complex (i.e., LFA-1 antigen) dependent on association of the constituent chains. Cytotoxic activity of NK cells in inhibited by antibodies MEM-25, MEM-95 and less strongly by MEM-30 and MEM-94.