The thiazolidinedione insulin sensitiser, BRL 49653, increases the expression of PPAR-gamma and aP2 in adipose tissue of high-fat-fed rats

• Published in: Biochemical and biophysical research communications Vol. 229; no. 3; pp. 752 - 757
• Main Authors: Pearson, S L, Cawthorne, M A, Clapham, J C, Dunmore, S J, Holmes, S D, Moore, G B, Smith, S A, Tadayyon, M
• Format: Journal Article
• Language: English
• Published: United States 24.12.1996
• Subjects: Adipose Tissue - metabolism; Animals; Carrier Proteins - biosynthesis; Dietary Fats - administration & dosage; Fatty Acid-Binding Protein 7; Fatty Acid-Binding Proteins; Hypoglycemic Agents - pharmacology; Male; Myelin P2 Protein - biosynthesis; Neoplasm Proteins; Nerve Tissue Proteins; Rats; Rats, Wistar; Receptors, Cytoplasmic and Nuclear - biosynthesis; RNA, Messenger - analysis; Thiazoles - pharmacology; Thiazolidinediones; Transcription Factors - biosynthesis
• ISSN: 0006-291X
• DOI: 10.1006/bbrc.1996.1876

The effects of the thiazolidinedione insulin sensitiser BRL 49653 on plasma leptin concentrations and on epididymal fat OB, PPAR-gamma and aP2 mRNA expression were examined in high-fat-fed and high-carbohydrate-fed adult Wistar rats. Diets were given for 4 weeks, with BRL 49653 (10 micromol/kg/day) administered by oral gavage for the last 4 days. Treatment with BRL 49653 reduced plasma leptin concentrations in high-fat-fed rats from 2.34 +/- 0.19 (n=9) to 1.42 +/- 0.09 (n=9) ng/ml (p<0.001). Plasma leptin was unaffected by BRL 49653 in the high-carbohydrate-fed rats. There was no difference in OB mRNA expression between high-fat-fed and high-carbohydrate-fed rats, with or without treatment. PPAR-gamma and aP2 mRNA expression were significantly increased in the high-fat-fed rats treated with BRL 49653 (p < 0.01 and p < 0.001 respectively), but not in carbohydrate-fed rats.