Immunoperoxidase for the detection of antibodies in cerebrospinal fluid in neurocysticercosis: use of Cysticercus cellulosae and Cysticercus longicollis particles fixed on microscopy slides

• Published in: Revista do Instituto de Medicina Tropical de São Paulo Vol. 38; no. 4; pp. 259 - 263
• Main Authors: de Andrade, A P, Vaz, A J, Nakamura, P M, Palou, V S, da Cunha, R A, Ferreira, A W
• Format: Journal Article
• Language: English
• Published: Brazil 01.07.1996
• Subjects: Animals; Antibodies, Helminth - cerebrospinal fluid; Antibodies, Helminth - isolation & purification; Cysticercosis - cerebrospinal fluid; Cysticercosis - diagnosis; Cysticercosis - immunology; Cysticercus - immunology; Female; Humans; Immunoenzyme Techniques; Mice; Mice, Inbred BALB C; Nervous System Diseases - cerebrospinal fluid; Nervous System Diseases - diagnosis; Nervous System Diseases - immunology; Nervous System Diseases - parasitology
• ISSN: 0036-4665
• DOI: 10.1590/s0036-46651996000400004

The ORF strain of Cysticercus longicollis represents an important model for the study of heterologous antigens in the immunodiagnosis of neurocysticcreosis (NC). The immunoperoxidase (IP) technique was standardized using a particulate antigen suspension of Cysticercus longicollis (Cl) and Cysitcercus cellulosae (Cc). Cerebrospinal fluid (CSF) samples were incubated on the antigen fixed to microscopy slides; the conjugate employed was anti-IgG-peroxidase and the enzymatic reaction was started by covering the slides with chromogen solution (diaminobenzidine/H2O2). After washing with distilled water, the slide was stained with 2% malachite green in water. Of the CSF samples from 21 patients with NC, 19 (90.5%) were positive, whereas the 8 CSF samples from the control group (100%) were negative. The results of the [P-C] test applied to 127 CSF samples from patients with suspected NC showed 28.3% reactivity as opposed to 29.1% for the IP-Cc test. The agreement index for the IP test (Cl x Cc) was 94.2%, with no significant difference between the two antigens.