Nutritional studies of Rickettsia guintana: nature of the hematin requirement

Rickettsia quintana grew in a liquid medium consisting of a brain-heart infusion base supplemented with starch and hematin. The growth requirement for hematin could not be substituted by compounds of known catalytic activity for H(2)O(2), viz., catalase, potassium pyruvate, or charcoal, or by the re...

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Bibliographic Details
Published inJournal of bacteriology Vol. 109; no. 1; p. 89
Main Authors Myers, W F, Osterman, J V, Wisseman, Jr, C L
Format Journal Article
LanguageEnglish
Published United States 01.01.1972
Subjects
Catalase - metabolism
Charcoal - metabolism
Culture Media
Escherichia coli - enzymology
Filtration
Heme - metabolism
Hydrogen Peroxide - metabolism
Pyruvates - metabolism
Rickettsia - enzymology
Rickettsia - growth & development
Rickettsia - metabolism
Sodium
Species Specificity
Spectrophotometry
Starch
Streptococcus pneumoniae - metabolism
Sulfites - metabolism
Temperature
Thioglycolates - metabolism
Time Factors
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Summary:Rickettsia quintana grew in a liquid medium consisting of a brain-heart infusion base supplemented with starch and hematin. The growth requirement for hematin could not be substituted by compounds of known catalytic activity for H(2)O(2), viz., catalase, potassium pyruvate, or charcoal, or by the reducing compounds sodium sulfite and sodium thioglycollate. R. quintana was catalase-negative, but no H(2)O(2) production could be demonstrated by the catalase-aminotriazole technique. A minimum inoculum giving 10(5) cells/ml was required to initiate growth. The generation time at 33 C was 10 hr. The temperature range for growth was 28 to 37 C. Growth was enhanced when succinate or glutamate was added as energy source.
ISSN:0021-9193