Purification and characterization of a thymocyte growth factor. 2. Biological activity of the thymocyte growth factor

A thymocyte growth factor, THGF, purified from the supernatant of a thymic T-lymphocyte-precursor cell line (TC.SC-1/2.0), stimulated the growth of splenocytes and thymocytes that had not been activated by a mitogen. Addition of suboptimal doses of mitogen or phorbol myristate acetate did not enhanc...

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Bibliographic Details
Published inBiomedical science Vol. 2; no. 5; p. 515
Main Authors Talaev VYu, Yarilin, A A, Sharova, N I, Shichkin, V P, Shuvaeva, T M
Format Journal Article
LanguageEnglish
Published England 1991
Subjects
Animals
Cell Division
Concanavalin A - pharmacology
Culture Media - pharmacology
Growth Substances - physiology
Mice
Mice, Inbred CBA
Peptides - physiology
Spleen - cytology
T-Lymphocytes - chemistry
T-Lymphocytes - cytology
Tetradecanoylphorbol Acetate - pharmacology
Thymosin - physiology
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Summary:A thymocyte growth factor, THGF, purified from the supernatant of a thymic T-lymphocyte-precursor cell line (TC.SC-1/2.0), stimulated the growth of splenocytes and thymocytes that had not been activated by a mitogen. Addition of suboptimal doses of mitogen or phorbol myristate acetate did not enhance the response. The growth factor could stimulate the growth of thymocytes synergistically with interleukin 2 but its action was not mediated by interleukin 2 or by interleukin 4. L3T4-Lyt2- thymocytes, Ig- splenocytes, Ig+ splenocytes, and alpha 1-thymosin-sensitive splenocytes were targets for THGF, which also stimulated the formation of haemopoietic spleen colonies in irradiated mice.
ISSN:0955-9701