Detection of chromosomal aberration using fluorescence in situ hybridization in DNA diploid colorectal carcinomas

Numerical aberrations of chromosomes can be detected by fluorescence in situ hybridization (FISH), using chromosome-specific probes. It is possible to observe this in solid tumors from which it is very difficult to obtain metaphase nuclei. The present study employed surgical specimens from 15 cases...

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Bibliographic Details
Published inGan to kagaku ryoho Vol. 20; no. 6; p. 759
Main Authors Jibiki, M, Tagawa, Y, Miyashita, K, Hara, S, Yasutake, T, Nakazaki, T, Obatake, M, Sawai, T, Morinaga, M, Akama, F
Format Journal Article
LanguageEnglish
Japanese
Published Japan 01.04.1993
Subjects
Chromosome Aberrations
Chromosomes, Human, Pair 11
Chromosomes, Human, Pair 17
Chromosomes, Human, Pair 7
Colorectal Neoplasms - genetics
DNA, Neoplasm - analysis
Female
Humans
In Situ Hybridization, Fluorescence
Male
Ploidies
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Summary:Numerical aberrations of chromosomes can be detected by fluorescence in situ hybridization (FISH), using chromosome-specific probes. It is possible to observe this in solid tumors from which it is very difficult to obtain metaphase nuclei. The present study employed surgical specimens from 15 cases of colorectal carcinoma, all of which showed DNA diploidy. In the same samples, we analyzed chromosomal numerical aberration by FISH according to the method of Pinkel et al. Biotinylated DNA probes specific to chromosome #7, #11 and #17, were used. The hybridization spots were observed by fluorescent microscopy. As a result, the numerical aberrations of chromosomes detected by FISH were found in DNA diploid cases by FCM. They were trisomy and monosomy. These results indicate that FISH is useful to detect the chromosomal aberrations of DNA diploid cases.
ISSN:0385-0684