Effect of anesthetics on membrane fluidity tested by two different techniques: EPR spin labels and polarization of perylene fluorescence

We have investigated the effect of n-butanol on the spectroscopic properties of two types of probes, that are usually employed to detect membrane fluidity, in mitochondrial phospholipid vesicles and mitochondrial membranes. Although both a spin label (16-doxylstearate) and the fluorescent probe pery...

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Bibliographic Details
Published inBollettino della Societa italiana di biologia sperimentale Vol. 56; no. 6; p. 527
Main Authors Curatola, G, Lenaz, G, Mazzanti, L, Grilli, G, Familiari, M
Format Journal Article
LanguageEnglish
Published Italy 30.03.1980
Subjects
Anesthetics - pharmacology
Animals
Butanols - pharmacology
Cattle
Cell Membrane Permeability - drug effects
Electron Probe Microanalysis
Electron Spin Resonance Spectroscopy
Fluorescence Polarization
In Vitro Techniques
Membrane Lipids
Membrane Proteins
Mitochondria, Heart - drug effects
Perylene
Phospholipids
Spin Labels
Stearic Acids
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Summary:We have investigated the effect of n-butanol on the spectroscopic properties of two types of probes, that are usually employed to detect membrane fluidity, in mitochondrial phospholipid vesicles and mitochondrial membranes. Although both a spin label (16-doxylstearate) and the fluorescent probe perylene detect a higher immobilization of the membrane in comparison with phospholipid vesicles, the effect of butanol in the case of the membrane is strikingly different with the two techniques employed, showing increased fluidity in the case of the spin label and decreased fluidity with the fluorescent probe.
ISSN:0037-8771