Effects of streptozotocin added in vitro on insulin biosynthesis and release in isolated rat islets

The effect of different concentrations of streptozotocin was tested in vitro on isolated rat islets. The following parameters of islet-cell activity were measured: 1) incorporation of 3H-leucine into proinsulin and into total islet protein; 2) IRI release into the medium, and 3) islet proinsulin con...

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Bibliographic Details
Published inHormone and metabolic research Vol. Suppl 6; p. 71
Main Authors Maldonato, A, Trueheart, P, Renold, A E
Format Journal Article
LanguageEnglish
Published Germany 1976
Subjects
Animals
In Vitro Techniques
Insulin - biosynthesis
Insulin - metabolism
Islets of Langerhans - drug effects
Islets of Langerhans - metabolism
Leucine - metabolism
Male
Proinsulin - biosynthesis
Rats
Streptozocin - pharmacology
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Summary:The effect of different concentrations of streptozotocin was tested in vitro on isolated rat islets. The following parameters of islet-cell activity were measured: 1) incorporation of 3H-leucine into proinsulin and into total islet protein; 2) IRI release into the medium, and 3) islet proinsulin content. It appears from the preliminary results shown here that both insulin release and biosynthesis were decreased by streptozotocin in a way which was dose- and time-dependent.
ISSN:0018-5043