Improved methods of influenza virus propagation. II. Characteristics of cell culture and allantoic virus preparations
Regular reproduction of influenza A virus with infectious titres of 7.4--8.5 log EID50/0.2 ml was observed in roller bovine embryo kidney cell cultures. The haemagglutinating activity of the cell culture preparations was 8-33-fold lower than that of the allantoic preparations, the infectivity being...
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Published in | Acta virologica (Anglickâa verze) Vol. 21; no. 3; p. 241 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Slovakia
01.05.1977
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Subjects | |
Online Access | Get more information |
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Summary: | Regular reproduction of influenza A virus with infectious titres of 7.4--8.5 log EID50/0.2 ml was observed in roller bovine embryo kidney cell cultures. The haemagglutinating activity of the cell culture preparations was 8-33-fold lower than that of the allantoic preparations, the infectivity being similar. Cell culture preparations of influenza A/Victoria/35/72 and A /Leningrad/538/74 viruses were markedly immunogenic in laboratory animals and antigenically active in complement fixation (CF) test. The CF activity of liquid and lyophilized preparations remained unchanged for 12--13 months (the period of observation). The stability of infectivity of lyophilized cell culture virus preparations was similar to that of allantoic virus preparations. |
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ISSN: | 0001-723X |