Improved methods of influenza virus propagation. II. Characteristics of cell culture and allantoic virus preparations

Regular reproduction of influenza A virus with infectious titres of 7.4--8.5 log EID50/0.2 ml was observed in roller bovine embryo kidney cell cultures. The haemagglutinating activity of the cell culture preparations was 8-33-fold lower than that of the allantoic preparations, the infectivity being...

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Bibliographic Details
Published inActa virologica (Anglickâa verze) Vol. 21; no. 3; p. 241
Main Authors Sominina, A A, Lisok, T P, Rumel, N B
Format Journal Article
LanguageEnglish
Published Slovakia 01.05.1977
Subjects
Allantois
Animals
Antibodies, Viral - biosynthesis
Antigens, Viral - analysis
Cattle
Chick Embryo
Complement Fixation Tests
Culture Techniques
Freeze Drying
Kidney
Orthomyxoviridae - growth & development
Orthomyxoviridae - immunology
Rabbits
Virus Cultivation - methods
Virus Replication
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Summary:Regular reproduction of influenza A virus with infectious titres of 7.4--8.5 log EID50/0.2 ml was observed in roller bovine embryo kidney cell cultures. The haemagglutinating activity of the cell culture preparations was 8-33-fold lower than that of the allantoic preparations, the infectivity being similar. Cell culture preparations of influenza A/Victoria/35/72 and A /Leningrad/538/74 viruses were markedly immunogenic in laboratory animals and antigenically active in complement fixation (CF) test. The CF activity of liquid and lyophilized preparations remained unchanged for 12--13 months (the period of observation). The stability of infectivity of lyophilized cell culture virus preparations was similar to that of allantoic virus preparations.
ISSN:0001-723X