Isolation, identification and phylogenetic analysis of a pathogen of Haliotis diversicolor supertexta (L.) with mass mortalities

This study was conducted to determine a disease outbreak in 14 day old post-larvae of abalone (Haliotis diversicolor supertexta) which caused mass mortality in July 2010 in Shanwei, China. Twenty-nine bacterial strains were isolated from a sample pool of 10 diseased post-larval abalones on 2216E mar...

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Bibliographic Details
Published inIranian journal of fisheries sciences Vol. 12; no. 4; pp. 843 - 854
Main Authors Zhou, Jing, Cai Junpeng
Format Journal Article
LanguageEnglish
Published 01.12.2013
Subjects
Freshwater
Haliotis diversicolor supertexta
China, Shanwei Province
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Summary:This study was conducted to determine a disease outbreak in 14 day old post-larvae of abalone (Haliotis diversicolor supertexta) which caused mass mortality in July 2010 in Shanwei, China. Twenty-nine bacterial strains were isolated from a sample pool of 10 diseased post-larval abalones on 2216E marine agar plates during a natural outbreak of the disease. Among them, a dominant isolate (referred to as strain 21) was found to be highly virulent to post-larvae in experimental challenge tests, with an LD50 value of 1.0 t10 super(4) colony forming units (CFU) mL super(-1) on day 3. API 20NE kits and 16S rDNA sequence analysis, identified strain 21 as Oceanomonas doudoroffii. It was susceptible to 10 and moderately susceptible to 1 of the 16 antibiotics examined when antibiotic sensitivities of the bacterium were assayed. Results of this study implicated Oceanomonas doudoroffii strain 21 as a cause of mortalities in post-larval abalone from Shanwei, China.
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ISSN:1562-2916