Colorimetric Determination of Lactate Dehydrogenase on Paper-Based Microwell Arrays Microfluidic Device

A low-cost, simple, sensitive detection method of lactate dehydrogense (LDH) was developed on paper-based microwell arrays microfluidic device. The phenazine methyl sulfate/nitrotetrazolium blue chloride (PMS/NBT) detection system was used for LDH detection and the colorimetric results were recorded...

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Bibliographic Details
Published inFēnxī huàxué Vol. 42; no. 9; pp. 1276 - 1280
Main Authors ZHANG, Hui-Yan, ZHANG, Zhen, JI, Xing-Hu, HE, Zhi-Ke
Format Journal Article
LanguageChinese
English
Published 01.09.2014
Subjects
Arrays
Cameras
Colorimetry
Devices
Documentation
Lactates
Mathematical analysis
Microfluidics
Reproducibility
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Summary:A low-cost, simple, sensitive detection method of lactate dehydrogense (LDH) was developed on paper-based microwell arrays microfluidic device. The phenazine methyl sulfate/nitrotetrazolium blue chloride (PMS/NBT) detection system was used for LDH detection and the colorimetric results were recorded by both Gel Documentation System and a common camera. Under the optimized conditions, the colorimetric intensity showed a linear correlation to the activity of LDH in the range of 10 to 150 U/L with a limit of detection (LOD) of 9.44 U/L (3[sigma]) by Gel Documentation System; and the linear range was 15-150 U/L by camera with a LOD of 12.36 U/L (3[sigma]). Foremost, it was found that human serum albumin (HSA) had an effect on the colorimetric enhancement in this detection system. This low-cost, portable paper-based analytical platform could be suitable for the application in the point-of-care with high sensitivity and reproducibility.
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ISSN:0253-3820
DOI:10.11895/j.issn.0253-3820.140331