Effects of artificial cell walls on syringyl-lignin growth reaction using recombinant cationic cell wall peroxidase

• Published in: Journal of wood science Vol. 71; no. 1; p. 52
• Main Authors: Matsuhisa, Naofumi, Tamura, Go, Kawaguchi, Natsumi, Fujita, Koki, Shigetomi, Kengo, Tsutsumi, Yuji
• Format: Journal Article
• Language: English
• Published: Singapore Springer Nature Singapore 01.12.2025; Springer Nature B.V
• Subjects: Alcohol; Biomedical and Life Sciences; Buffers; Cations; Cellulose; Characterization and Evaluation of Materials; Dehydrogenation; Esterification; Ferulic acid; Fourier transforms; Life Sciences; Lignin; Materials Science; Microscopy; Molecular weight; Original Article; Peroxidase; Polymerization; Polymers; Polysaccharides; Wood Science & Technology; rCWPO-C; Xyloglucan; Sinapyl alcohol; Artificial cell wall; Β-O-4; Dehydrogenative polymers (DHPs); Ferulic acid
• ISSN: 1435-0211; 1611-4663
• DOI: 10.1186/s10086-025-02224-x

Artificial lignin called dehydrogenative polymers (DHPs) has been used to develop models for investigating lignin characteristics and structures. However, these models are inadequate because they have lower molecular weight and less content of β- O -4 than natural lignin. The conditions required for dehydrogenative polymerization, particularly the reaction within the polysaccharide matrix, are believed to be critical for facilitating lignin polymer growth reaction. This study investigated the effects of the polysaccharide matrix on syringyl-DHPs properties following dehydrogenative polymerization reaction on artificial cell walls. Specifically, artificial cell walls were prepared by esterifying ferulic acid to xyloglucan, which was then adsorbed on nematic ordered cellulose film. Sinapyl alcohol or coniferyl alcohol was polymerized on the film using recombinant cationic cell wall peroxidase. Syringyl-DHP and guaiacyl-DHP were prepared in the buffer and served as comparative subjects. DHPs formed on artificial cell walls had lower molecular weights and less content of β- O -4 than DHPs prepared in the buffer. These results suggest that binding DHPs to a polysaccharide matrix may limit their mobility and inhibit their growth reaction.