Selective Identification of V alpha 14i T Cells Using Slide-Immobilized, CD1d-Antigen Complexes

The ability to correlate changes in antigen-reactive lymphocytes with disease will provide information needed to develop strategies for combating illness. One critical group of lymphocytes are the CD1-restricted T cells. It is desirable to use CD1 molecules in an array format to query CD1-restricted...

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Bibliographic Details
Published inJournal of immunoassay & immunochemistry Vol. 27; no. 3; pp. 207 - 212
Main Authors Zullo, A J, Brutkiewicz, R R, Taparowsky, E J
Format Journal Article
LanguageEnglish
Published 01.07.2006
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Summary:The ability to correlate changes in antigen-reactive lymphocytes with disease will provide information needed to develop strategies for combating illness. One critical group of lymphocytes are the CD1-restricted T cells. It is desirable to use CD1 molecules in an array format to query CD1-restricted lymphocytes in humans. To investigate the feasibility of this technique, we employed mCD1d and alpha -galactosylceramide to demonstrate that-slide immobilized, CD1d- alpha -GalCer complexes capture an NKT cell hybridoma in the presence of a competitor. The success of this scheme represents the first step toward the development of CD1-antigen arrays that could be used to profile biological samples.
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ISSN:1532-1819
DOI:10.1080/15321810600734893