Effects of different biopsy methods on the development of preimplantation mouse embryos, in vitro and in vivo: implication for preimplantation genetic diagnosis

• Published in: Journal of the Medical Association of Thailand Vol. 82; no. 11; p. 1142
• Main Authors: Tangchaisin, P, Pruksananonda, K, Tirawatnapong, T, Yodyingyuad, V, Virutamasen, P
• Format: Journal Article
• Language: English
• Published: Thailand 01.11.1999
• Subjects: Animals; Biopsy, Needle - methods; Blastocyst; Female; Mice; Mice, Inbred ICR; Pregnancy; Preimplantation Diagnosis; Zona Pellucida
• ISSN: 0125-2208

The aim of this study was to investigate the efficacy of two different embryo biopsy techniques, direct aspiration and partial zona dissection (PZD)-push, on subsequent in vitro and in vivo development of 8-cell stage mouse embryos. It was found that the rates of normal blastocyst formation and hatching blastocysts of direct aspiration, PZD-push, solution control and control embryos were not significantly different (80.8%, 81.6%, 84.5%, 86.7% and 71.9%, 72.3% and 74.6%) respectively. There was, however, a significant reduction in rate of complete hatching blastocysts (P < 0.1) (72.9% aspiration versus 85.2 per cent solution control and 86.4% control) and rate of live-born fetuses (24.2% aspiration versus 43.3% solution control and 41.2% control) (P < 0.05) in the direct aspiration group but no significant difference in the PZD-push group (80.3% of complete hatched blastocysts and 33.8% of live-born fetuses). These findings indicated that embryo biopsy with PZD-push was superior to the direct aspiration method. This mouse embryo biopsy model was useful in advancing development of biopsy technique for human preimplantation genetic diagnosis.