Adrenergic and serotoninergic mechanisms in human hand arteries and veins studied by fluorescence histochemistry and in vitro pharmacology

Isolated hand arteries and veins from healthy human subjects were tested in vitro for their contractile response to adrenergic agonists and 5-hydroxytryptamine (5-HT) under standardized conditions. This allowed for quantitative estimation of various receptor characteristics. The relative sympathomim...

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Bibliographic Details
Published inBlood vessels Vol. 22; no. 1; p. 1
Main Authors Arneklo-Nobin, B, Owman, C
Format Journal Article
LanguageEnglish
Published Switzerland 1985
Subjects
Arteries - analysis
Arteries - innervation
Binding, Competitive
Biogenic Amines - analysis
Epinephrine - pharmacology
Female
Hand - blood supply
Humans
Isoproterenol - pharmacology
Ketanserin
Male
Microscopy, Fluorescence
Muscle Contraction - drug effects
Norepinephrine - analysis
Phentolamine - pharmacology
Piperidines - pharmacology
Receptors, Adrenergic - metabolism
Serotonin - pharmacology
Serotonin - physiology
Sympathetic Nervous System - physiology
Veins - analysis
Veins - innervation
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Summary:Isolated hand arteries and veins from healthy human subjects were tested in vitro for their contractile response to adrenergic agonists and 5-hydroxytryptamine (5-HT) under standardized conditions. This allowed for quantitative estimation of various receptor characteristics. The relative sympathomimetic potency suggested alpha-adrenergic receptors, which was confirmed in Schild plots following phentolamine antagonism of the response (pA2 for artery 7.57, for vein 7.75). 5-HT contracted with a relative potency approximately equal to noradrenaline and adrenaline in arteries, but only one fifth to one tenth of the catecholamine activity in veins. Ketanserin inhibited the 5-HT response in a competitive, probably also irreversible, manner in arteries (pA2 9.50, KA 8.90 X 10(-7) M). In the veins, ketanserin counteracted the 5-HT-induced contraction in a noncompetitive way.
ISSN:0303-6847