Enemies join forces for gene insertion

[...]transposons themselves are more-efficient tools for gene addition5, because they encode a transposase enzyme that catalyses movement of transposon DNA to new genomic sites6. [...]TnsB might not be able to bind to natural target DNA in the absence of TnsC, which tethers it to DNA. [...]in the st...

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Bibliographic Details
Published inNature (London) Vol. 613; no. 7945; pp. 1 - 2
Main Authors Barabas, Orsolya, Rice, Phoebe A
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group 26.01.2023
Subjects
Biochemistry
Biology
Cooperation
CRISPR
Deoxyribonucleic acid
DNA
DNA structure
Genomes
Proteins
Transposase
Transposons
United States > US
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Summary:[...]transposons themselves are more-efficient tools for gene addition5, because they encode a transposase enzyme that catalyses movement of transposon DNA to new genomic sites6. [...]TnsB might not be able to bind to natural target DNA in the absence of TnsC, which tethers it to DNA. [...]in the structure reported by Park et al., the TnsC minifilament is positioned to contact target DNA on both sides of TnsB, perhaps to stabilize its bent form. [...]the authors observed that interactions between TnsB and TnsC caused a disordered segment of TnsB to fold and dock in a way that might help to stabilize bent target DNA. [...]the CAST system could potentially be used as a blueprint to establish programmable targeting of simpler transposons.
ISSN:0028-0836
1476-4687
DOI:10.1038/d41586-022-04584-6