TGF-β1 promotes human gastric carcinoma SGC7901 cells invasion by inducing autophagy

To investigate the role of TGF-b1 in autophagy and invasion ability in human gastric carcinoma cell line SGC7901. Cultured SGC7901 cells were treated with different concentrations of TGF-b1 for 24 h. The protein expression levels of autophagy relative marker LC3 and Beclin1 were detected by Western...

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Bibliographic Details
Published inEuropean review for medical and pharmacological sciences Vol. 21; no. 5; p. 1013
Main Authors Shen, J, Zhao, D-S, Li, M-Z
Format Journal Article
LanguageEnglish
Published Italy 01.03.2017
Subjects
Autophagy - drug effects
Cell Line, Tumor
Cell Movement - drug effects
Humans
Neoplasm Invasiveness
Stomach Neoplasms
Transforming Growth Factor beta1 - metabolism
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Summary:To investigate the role of TGF-b1 in autophagy and invasion ability in human gastric carcinoma cell line SGC7901. Cultured SGC7901 cells were treated with different concentrations of TGF-b1 for 24 h. The protein expression levels of autophagy relative marker LC3 and Beclin1 were detected by Western blot. The effect of TGF-b1 on invasion ability of SGC7901 cells was detected with transwell method. The results demonstrated that TGF-b1 was able to induced autophagy of SGC7901 cells in a dose-dependent manner. Autophagy inhibitor 3-MA could inhibit TGF-b1 upregulated autophagy. Furthermore, TGF-b1 significantly enhances the invasion ability of SGC7901 cells. However, autophagy inhibitor 3-MA could effectively reverse this process. TGF-b1 enhances SGC7901 cells migration by inducing autophagy.
ISSN:2284-0729