Maribellus luteus gen. nov., sp. nov., a marine bacterium in the family Prolixibacteraceae isolated from coastal seawater

• Published in: International journal of systematic and evolutionary microbiology Vol. 69; no. 8; p. 2388
• Main Authors: Zhou, Liu-Yan, Yu, Zi-Liang, Xu, Wei, Mu, Da-Shuai, Du, Zong-Jun
• Format: Journal Article
• Language: English
• Published: England 01.08.2019
• Subjects: Bacterial Typing Techniques; Bacteroidetes - classification; Bacteroidetes - isolation & purification; Base Composition; China; DNA, Bacterial - genetics; Fatty Acids - chemistry; Islands; Phosphatidylethanolamines - chemistry; Phylogeny; Pigmentation; RNA, Ribosomal, 16S - genetics; Seawater - microbiology; Sequence Analysis, DNA; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - chemistry; China; Prolixibacteraceae; dDDH; Maribellus luteus; draft genome sequencing
• ISSN: 1466-5034
• DOI: 10.1099/ijsem.0.003495

A facultatively anaerobic, Gram-stain-negative, non-motile and straight to slightly curved long rod-shaped bacterial strain, designated XSD2 , was isolated from coastal seawater of Xiaoshi Island, PR China. The cells were catalase-positive, oxidase-negative and non-flagellated. Strain XSD2 was found to grow at 20-40 °C (optimum, 33 °C), at pH 6.0-8.5 (pH 7.0-7.5) and with 1-5 % (w/v) NaCl (3 %). Carotenoid pigments were produced. The major cellular fatty acids (>10.0 %) were iso-C15 : 0, iso-C16 : 0 3-OH and C17 : 1ω6c and the major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and three unidentified polar lipids. The sole respiratory quinone was MK-7 and the genomic DNA G+C content was 44.1 mol%. The result of the 16S rRNA gene sequence analysis confirmed the affiliation of this organism to the order Marinilabiliales, family Prolixibacteraceae, with Mariniphaga sediminis SY21 as its closest relative with only 93.6 % sequence similarity. On the basis of physiological, biochemical and chemotaxonomic characteristics, we propose that strain XSD2 (=KCTC 62994 =MCCC 1H00347 ) represents a novel species of a novel genus in the family Prolixibacteraceae, for which the name Maribellus luteus gen. nov., sp. nov. is proposed.