MiR-30 suppresses lung cancer cell 95D epithelial mesenchymal transition and invasion through targeted regulating Snail

• Published in: European review for medical and pharmacological sciences Vol. 21; no. 11; p. 2642
• Main Authors: Fan, M-J, Zhong, Y-H, Shen, W, Yuan, K-F, Zhao, G-H, Zhang, Y, Wang, S-K
• Format: Journal Article
• Language: English
• Published: Italy 01.06.2017
• Subjects: 3' Untranslated Regions - genetics; Carcinoma, Non-Small-Cell Lung - genetics; Carcinoma, Non-Small-Cell Lung - pathology; Cell Line, Tumor; Down-Regulation; Epithelial-Mesenchymal Transition - genetics; Female; HEK293 Cells; Humans; Lung Neoplasms - genetics; Lung Neoplasms - pathology; MicroRNAs - genetics; Neoplasm Invasiveness; Snail Family Transcription Factors - genetics; Transfection; Up-Regulation
• ISSN: 2284-0729

As an important factor regulating the epithelial mesenchymal transition (EMT) Snail is associated with lung cancer. Bioinformatics analysis showed that microRNA-30a (miR-30a) may target the 3'-UTR of Snail mRNA. It was exhibited that miR-30a down-regulation was related to tumor size, TNM stage, and poor prognosis of non-small cell lung cancer (NSCLC) patients, which suggests that miR-30a might participate in NSCLC attack. This study aims to explore the role of miR-30a and Snail in NSCLC invasion and metastasis. NSCLC tumor and para-carcinoma tissues were collected from 46 patients to evaluate the miR-30a and Snail expressions. The targeted relationship between miR-30a and Snail was verified by using dual-luciferase reporter assay. 95D cells were cultured in vitro and transfected with miR-30a mimic or small interfere RNA targeting Snail (si-Snail). The expression of miR-30a, Snail, EMT-related factors, malignant growth, invasion, and apoptosis, were compared. Snail was significantly up-regulated, while miR-30a was significantly reduced in NSCLC tissue. MiR-30a suppressed Snail expression by targeting the 3'-URT of Snail mRNA. 95D cells exhibited significantly higher Snail, N-cadherin, and vimentin levels, while lower miR-30a, E-cadherin, and occludin expressions were compared with 95C cells. 95D cells presented stronger malignant growth and invasive ability, whereas lower background apoptosis than 95C. MiR-30a mimic and/or si-Snail transfection significantly enhanced E-cadherin and occludin expression, while significantly declined N-cadherin and vimentin levels, thus weakening malignant growth and invasion and increasing cell apoptosis. Snail up-regulated, while miR-30a declined in NSCLC tissue. MiR-30a may suppress Snail expression, restrain EMT, and inhibit lung cancer cell invasion.