Effects of tumor necrosis factor and E-selectin on coronary artery flow

• Published in: European review for medical and pharmacological sciences Vol. 21; no. 8; p. 1843
• Main Authors: Liu, W-B, Han, X-H, Guo, Y-Y, Zhang, D-M, Tang, F-J, Zhao, L, Ji, L-L, Guo, F-M
• Format: Journal Article
• Language: English
• Published: Italy 01.04.2017
• Subjects: Animals; Coronary Vessels - physiopathology; Drugs, Chinese Herbal - pharmacology; E-Selectin - metabolism; Myocardial Ischemia - blood; Myocardial Ischemia - physiopathology; Regional Blood Flow; Swine; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 2284-0729

The aim of the study is to investigate the correlation between tumor necrosis factor (TNF-α), E-selectin and coronary artery flow following myocardial ischemia-reperfusion model (IR) in Yorkshire pigs. Establishment of IR model in pigs. Following the injury model, Experiment group was administrated intravenously Shenfu injection solution (SFI, 1 mL/kg). The control group received the same amount of saline. After 30 min of blood reflux, thrombolysis in myocardial infarction frame count (TFC) was recorded following surgery. TNF-α, E-selectin expression was determined by ELISA in the venous sheath, coronary sinus, artery sinus, and proximal segment of the coronary artery. After the blood reflowing, TFC in both groups were upregulated, and TFC increased more than the control group. The difference is statistically significant (p<0.05) at the time of 30 min. TNF-α, E-selectin expression increased after IR. After reperfusion, TNF-α, E-selectin levels further increased and the myocardial injury was aggravated. SFI inhibited inflammation in the experimental group. TNF-α, E-selectin levels at coronary sinus, artery sinus, and distal segment of coronary artery after surgery was positively correlated with TIMI in the experimental group (p<0.05). TNF-α, E-selectin levels significantly increased after reperfusion (p<0.05). The result demonstrated that TNF-α, E-selectin levels were positively correlated with coronary artery reflow only in the experimental group but not in the control group.