Recent trends in determination of thiamine and its derivatives in clinical practice

Over the last five decades, many methods to analyze thiamine (vitamin B ) and its phosphorylated forms in urine, whole blood, serum, plasma and erythrocytes have been proposed. Some of the methods are presently used in routine practice, but analytical problems regarding reproducibility, standardizat...

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Bibliographic Details
Published inJournal of chromatography. A Vol. 1510; p. 1
Main Authors Jenčo, Jaroslav, Krčmová, Lenka Kujovská, Solichová, Dagmar, Solich, Petr
Format Journal Article
LanguageEnglish
Published Netherlands 11.08.2017
Subjects
Chemistry, Clinical - standards
Chemistry, Clinical - trends
Erythrocytes - chemistry
Humans
Reference Standards
Reproducibility of Results
Thiamine - analysis
Thiamine - blood
Thiamine - urine
Sample preparation
Bioanalysis
Vitamin B
Blood
Chromatography
Thiamine
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Summary:Over the last five decades, many methods to analyze thiamine (vitamin B ) and its phosphorylated forms in urine, whole blood, serum, plasma and erythrocytes have been proposed. Some of the methods are presently used in routine practice, but analytical problems regarding reproducibility, standardization, lack of automation, time consuming procedures for pretreatment and analysis are often discussed. With modern approaches to bioanalysis in clinical research of vitamins, whole processes can be automated, making analysis less time consuming, with reduced consumption of solvents and samples. This review critically discusses various analytical techniques, their advantages and disadvantages that are used for determination of thiamine and its derivatives in clinical practice, with emphasis on accurate, reliable and fast analytical procedures.
ISSN:1873-3778
DOI:10.1016/.j.chroma.2017.06.048