Effect of Prostaglandin E 1 Injected Into Donors in a Heterotopic Heart Transplant Model of Sprague Dawley Rats

• Published in: Transplantation proceedings Vol. 51; no. 8; p. 2808
• Main Authors: Cho, Minji, Kim, Wan-Seop, Shin, Hyesun, Yun, Ik-Jin
• Format: Journal Article
• Language: English
• Published: United States 01.10.2019
• Subjects: Alprostadil - administration & dosage; Alprostadil - therapeutic use; Animals; Heart Transplantation - adverse effects; Male; Rats; Rats, Sprague-Dawley; Reperfusion Injury - etiology; Reperfusion Injury - prevention & control; Tissue Donors; Transplantation, Heterotopic; Vasodilator Agents - administration & dosage; Vasodilator Agents - therapeutic use
• ISSN: 1873-2623
• DOI: 10.1016/j.transproceed.2019.01.198

Prostaglandin E (PGE ) administered to patients in the immediate post-transplant period has been known to reduce ischemic reperfusion injuries (IRIs), but the effect on IRI of PGE administered to the donor is unknown. The purpose of this study was to determine the effect on IRI of PGE injected into donor rats during heterotopic heart transplantation. Genetically identical male Sprague Dawley rats with a body weight of 300-320 g at 8-9 weeks of age were used for the study. Experimental methods were the same in the control (G , n = 6) and experimental groups (G , n = 6), but only the donor rats in the experimental group received an intramuscular injection of PGE (5 μg/kg) prior to the donor surgery. On day 1 the animals were sacrificed with the removal of the transplanted heart. Histologic analysis was performed in the hematoxylin-eosin-stained slides to assess interstitial edema and neutrophil infiltration by a pathologist. Median times of the donor organ procurement, cold ischemia, and warm ischemia were 37, 69, and 35 minutes, respectively, in the G group and 38, 76.5, and 33 minutes respectively in G group; there were no statistical differences. Heartbeats were observed in the transplanted graft in 2 of the G group and 2 of G group immediately after heart transplantation, but in all transplanted grafts on day 1 after surgery. Histologic scores for neutrophil infiltration showed significantly lower in the G group than in the G group. PGE administration to donors in a rat heart transplantation model may significantly reduce IRI.