Determination of the native forms of vitamin B 1 in bovine milk using a fast and simplified UHPLC method

• Published in: Food chemistry Vol. 229; p. 452
• Main Authors: Schmidt, Anatol, Pratsch, Herbert, Schreiner, Maximilian G, Mayer, Helmut K
• Format: Journal Article
• Language: English
• Published: England 15.08.2017
• Subjects: Animals; Chromatography, High Pressure Liquid - methods; Milk - chemistry; Reproducibility of Results; Thiamine - analysis; Thiamin diphosphate (PubChem CID: 15938963); Thiamin phosphate (PubChem CID: 3382778); UHPLC; Thiamin (PubChem CID: 1130); Thiamin phosphate esters; Thiamin; Milk
• ISSN: 0308-8146
• DOI: 10.1016/j.foodchem.2017.02.092

The aim of this study was to develop a high-throughput UHPLC method for the determination vitamin B active compounds; thiamin, thiamin monophosphate and thiamin diphosphate in bovine milk. In order to sustain the native vitamin B phosphorus esters, sample preparation is crucial. Various acids as well as commonly used enzymes and their enzyme mixtures were compared. Method accuracy was confirmed using certified reference material as well as comparison with the corresponding CEN method, and was found to be satisfactory. Studied milk samples showed significant amounts of thiamin monophosphate, which can make up to 53.9% of the total vitamin B content in commercial milk, and up to 78% in raw milk. Moreover, a tremendous variation of the total content of vitamin B was observed between single cows, which ranged from 0.24mg/L up to 0.54mg/L of total vitamin B .