"Diaminobenzidine black" as a new histochemical demonstration of exogenous iron (author's transl)

A new reaction for the histochemical demonstration of exogenous iron, used as a tracer for the study of connectivity in the central nervous system, is described. It consists of, first, the conversion of iron into Prussian blue, which acts secondarily as a catalyst for the oxidation of diaminobenzidi...

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Bibliographic Details
Published inHistochemistry (Berlin) Vol. 66; no. 3; p. 239
Main Authors Nguyen-Legros, J, Bizot, J, Bolesse, M, Pulicani, J P
Format Journal Article
LanguageFrench
Published Germany 1980
Subjects
3,3'-Diaminobenzidine
Animals
Benzidines
Catalysis
Central Nervous System - metabolism
Histocytochemistry
Hydrogen Peroxide - pharmacology
Iron - analysis
Iron - pharmacology
Oxidation-Reduction
Polarography
Prussian Blue Reaction
Rats
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Summary:A new reaction for the histochemical demonstration of exogenous iron, used as a tracer for the study of connectivity in the central nervous system, is described. It consists of, first, the conversion of iron into Prussian blue, which acts secondarily as a catalyst for the oxidation of diaminobenzidine by hydrogen peroxide. The oxidized diaminobenzidine precipitates by polymerization and gives rise to a brown-colored insoluble reaction product. This reaction has a strikingly better sensitivity and contrast than the classical Perls's reaction.
ISSN:0301-5564
DOI:10.1007/BF00495737