"Diaminobenzidine black" as a new histochemical demonstration of exogenous iron (author's transl)
A new reaction for the histochemical demonstration of exogenous iron, used as a tracer for the study of connectivity in the central nervous system, is described. It consists of, first, the conversion of iron into Prussian blue, which acts secondarily as a catalyst for the oxidation of diaminobenzidi...
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Published in | Histochemistry (Berlin) Vol. 66; no. 3; p. 239 |
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Main Authors | , , , |
Format | Journal Article |
Language | French |
Published |
Germany
1980
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Subjects | |
Online Access | Get more information |
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Summary: | A new reaction for the histochemical demonstration of exogenous iron, used as a tracer for the study of connectivity in the central nervous system, is described. It consists of, first, the conversion of iron into Prussian blue, which acts secondarily as a catalyst for the oxidation of diaminobenzidine by hydrogen peroxide. The oxidized diaminobenzidine precipitates by polymerization and gives rise to a brown-colored insoluble reaction product. This reaction has a strikingly better sensitivity and contrast than the classical Perls's reaction. |
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ISSN: | 0301-5564 |
DOI: | 10.1007/BF00495737 |