Transduction of oncogene RAS into antibody-producing hybridoma

The effect of introduction by means of electroporation of protogene ras into cells of antibody-producing hybridoma was studied. Doubling time (TD) of transduced subclones, in which H-ras gene was expressed, proved to be 1.8-fold shorter as compared to the parent line. Other characteristics of the hy...

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Bibliographic Details
Published inGematologiia i transfuziologiia Vol. 36; no. 8; p. 7
Main Authors Belkina, E V, Deriugina, E I, Drize, N I, Olovnikova, N I, Sadovnikova, E Iu, Chertkov, I L
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.08.1991
Subjects
Animals
Antibodies, Monoclonal - biosynthesis
Biotechnology - methods
Cells, Cultured
Gene Expression - genetics
Gene Expression - physiology
Genes, ras - genetics
Genes, ras - physiology
Hybridomas - cytology
Hybridomas - immunology
In Vitro Techniques
Mice
Mice, Inbred BALB C
Multiple Myeloma - pathology
Oncogenes - genetics
Oncogenes - physiology
Plasmids - genetics
Spleen - cytology
Transduction, Genetic - genetics
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Summary:The effect of introduction by means of electroporation of protogene ras into cells of antibody-producing hybridoma was studied. Doubling time (TD) of transduced subclones, in which H-ras gene was expressed, proved to be 1.8-fold shorter as compared to the parent line. Other characteristics of the hybridoma (cloning efficiency, antibody production in vitro, the time of ascitic fluid appearance in mice and antibody titer in vivo) were not changed. Addition of 12-0-tetradecanoylphorbol acetate to the culture did not alter the growth parameters of H-ras transduced subclones.
ISSN:0234-5730